1ECE

ACIDOTHERMUS CELLULOLYTICUS ENDOCELLULASE E1 CATALYTIC DOMAIN IN COMPLEX WITH A CELLOTETRAOSE

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	97.130, 97.130, 258.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.179
Rwork	0.179
R-free	0.23600
RMSD bond length	0.007
RMSD bond angle	1.200 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.400
Rmerge	0.092 *
Number of reflections	47386
Completeness [%]	90.0
Redundancy	2.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	E1cd	11.4 (mg/ml)
2	1	drop		2.3 (M)
3	1	drop	cellobiose	0.3 (M)
4	1	drop	citrate	0.1 (M)