1ECC
ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH MN-CPRPP AND 5-OXO-NORLEUCINE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.200, 78.200, 308.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.100 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | HKL |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.036 * | 0.172 * |
| Total number of observations | 99285 * | |
| Number of reflections | 27331 | |
| <I/σ(I)> | 22.7 | 3.1 |
| Completeness [%] | 88.3 | 60.3 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.8 | 20 * | 0.42MM CPRPP, 25MM MNCL2, 40MM BIS-TRIS PH 5.8, 3.2% 2-PROPANOL, 7.5% PEG-3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.6 (mg/ml) | |
| 2 | 1 | drop | cPRPP | 0.42 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | Bis-Tris | 40 (mM) | |
| 5 | 1 | drop | 2-propanol | 3.2 (%) | |
| 6 | 1 | drop | PEG3350 | 7.5 (%) | |
| 7 | 1 | reservoir | bis-Tris | 100 (mM) | |
| 8 | 1 | reservoir | 2-propanol | 8 (%) | |
| 9 | 1 | reservoir | PEG3350 | 15 (%) |
