1ECB
ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.800, 113.200, 199.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.700 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.400 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | HKL |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.092 * | 0.330 * |
| Total number of observations | 354923 * | |
| Number of reflections | 58955 | |
| <I/σ(I)> | 12.7 | 3.5 |
| Completeness [%] | 97.7 | 78.9 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 5 MM GMP, 20 MM MGCL2, 100 MM BIS-TRIS PH 6.0, 10% PEG-3350, 10% 2-PROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 10 | 1 | reservoir | 20 (mM) | ||
| 11 | 1 | reservoir | D,L-meso-2,3-butanediol | 18 (%) | |
| 12 | 1 | reservoir | GMP | 1 (mM) | |
| 2 | 1 | drop | GMP | 2.5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | Bis-Tris | 50 (mM) | |
| 5 | 1 | drop | PEG3350 | 5 (%) | |
| 6 | 1 | drop | 2-propanol | 5 (%) | |
| 7 | 1 | reservoir | bis-Tris | 100 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 10 (%) | |
| 9 | 1 | reservoir | 2-propanol | 10 (%) |
