1EC7
E. COLI GLUCARATE DEHYDRATASE NATIVE ENZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-02-11 |
Detector | OTHER |
Spacegroup name | P 1 |
Unit cell lengths | 71.250, 84.550, 98.920 |
Unit cell angles | 103.30, 94.00, 113.10 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.194 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.700 | 1.900 |
Rmerge | 0.070 | 0.202 |
Total number of observations | 526735 * | |
Number of reflections | 150262 * | |
<I/σ(I)> | 8.1 | |
Completeness [%] | 95.2 | 90 |
Redundancy | 2.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 8 | 277 | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG5000 ME | 14 (%) | |
2 | 1 | 1 | 75 (mM) | ||
3 | 1 | 1 | 2-propanol | 5 (%) | |
4 | 1 | 1 | HEPPES | 50 (mM) |