1EC6

CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN BOUND TO 20-MER RNA HAIRPIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09-01
Detector	OTHER
Spacegroup name	P 21 21 21
Unit cell lengths	47.850, 51.210, 128.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.208 *
Rwork	0.208
R-free	0.29100
RMSD bond length	0.008 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.081	0.211 *
Total number of observations	143241 *
Number of reflections	67678 *
<I/σ(I)>	16.9
Completeness [%]	98.0 *	93.7
Redundancy	5.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	20 *	protein:RNA 1:1.25 ratio *

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MES
2	1	1	(NH4)2SO4
3	1	1	PEG 5000
4	1	2	(NH4)2SO4
5	1	2	PEG 5000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	25 (%)
2	1	reservoir	ammonium sulfate	150 (mM)
3	1	reservoir	MES	100 (mM)	pH6.5
4	1	drop	protein	0.155 (mM)