1EBL
THE 1.8 A CRYSTAL STRUCTURE AND ACTIVE SITE ARCHITECTURE OF BETA-KETOACYL-[ACYL CARRIER PROTEIN] SYNTHASE III (FABH) FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-21 |
Detector | OTHER |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.130, 64.300, 165.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22500 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.600 | 1.890 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 0.140 |
Total number of observations | 230489 * | |
Number of reflections | 61752 | |
<I/σ(I)> | 20.52 | |
Completeness [%] | 97.4 | 89.8 |
Redundancy | 3.73 | 2.88 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | 1.8-2.0 M ammonium sulphate, 2% PEG 400, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) | |
6 | 1 | reservoir | PEG400 | 2 (%) | |
7 | 1 | reservoir | HEPES | 100 (mM) |