1EBG

CHELATION OF SER 39 TO MG2+ LATCHES A GATE AT THE ACTIVE SITE OF ENOLASE: STRUCTURE OF THE BIS(MG2+) COMPLEX OF YEAST ENOLASE AND THE INTERMEDIATE ANALOG PHOSPHONOACETOHYDROXAMATE AT 2.1 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	123.500, 73.900, 94.800
Unit cell angles	90.00, 93.30, 90.00

Refinement procedure

Resolution	? - 2.100
R-factor	0.186
RMSD bond length	0.015 *
RMSD bond angle	2.100 *
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.047 *	0.166 *
Total number of observations	84862 *
Number of reflections	38673 *	2799 *
Completeness [%]	77.0 *	40 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	32 (%(w/w))
2	1	1		0.5 (M)
3	1	1	HEPPS-NaOH	80 (mM)