1EBD

DIHYDROLIPOAMIDE DEHYDROGENASE COMPLEXED WITH THE BINDING DOMAIN OF THE DIHYDROLIPOAMIDE ACETYLASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	106.600, 106.600, 204.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.215
Rwork	0.215
RMSD bond length	0.010
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.600 *	2.640 *
Rmerge	0.092 *
Number of reflections	44969
Completeness [%]	95.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9-10 (mg/ml)
2	1	reservoir	PEG600	12 (%)
3	1	reservoir	zinc acetate	200 (mM)
4	1	reservoir	FAD	0.001 (mM)