1EBC

SPERM WHALE MET-MYOGLOBIN:CYANIDE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	64.870, 31.000, 35.290
Unit cell angles	90.00, 105.63, 90.00

Refinement procedure

Resolution	15.300 - 1.800
R-factor	0.181 *
Structure solution method	OTHER
RMSD bond length	0.014
RMSD bond angle	15.373 *
Data reduction software	MOSFLM
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.340
High resolution limit [Å]	1.700	1.800 *
Rmerge	0.045 *
Number of reflections	14099
Completeness [%]	97.0
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.8 *	4 *	Rizzi, M., (1994) J. Mol. Biol., 244, 86. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	acetate	0.05 (M)
3	1	drop	ammonium sulfate	1.3 (M)
4	1	reservoir	ammonium sulfate	2.6 (M)