1EB6
Deuterolysin from Aspergillus oryzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.430, 34.764, 60.276 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 57.730 - 1.000 |
| R-factor | 0.103 * |
| Rwork | 0.104 |
| R-free | 0.12600 |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.0.36) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.052 | 0.240 |
| Number of reflections | 82199 | |
| <I/σ(I)> | 23 | 5 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.5 | 3.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 291 * | 1.8M NA,K PHOSPHATE, 0.1M HEPES PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | sodium potassium phosphate | 1.8 (M) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
