1EB3
YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 4,7-DIOXOSEBACIC ACID COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-29 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 104.300, 104.300, 167.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.300 - 1.750 |
| R-factor | 0.239 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.021 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.114 | 0.364 |
| Number of reflections | 44379 | |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 95.5 | 98.5 |
| Redundancy | 6.2 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | AS FOR PDB ENTRY 1AW5 WITH 5 MM 4,7-DIOXO-SEBACIC ACID IN DROP, pH 8.00 |
