1EB2
Trypsin inhibitor complex (BPO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.080, 63.825, 70.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ptn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.059 |
| Number of reflections | 17272 |
| Completeness [%] | 91.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 2.1 M AMMONIUM SULPHATE, 0.05 M TRIS PH 8.15 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 0.05 (M) | pH8. |
| 3 | 1 | drop | 3 (mM) | ||
| 4 | 1 | drop | acetonitrile | 18 (%) | |
| 5 | 1 | drop | DMF | 5 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 7 | 1 | reservoir | Tris | 0.05 (M) | pH8.15 |
