1EAV
Crystal Structures of Human Gephyrin and Plant Cnx1 G domains - Comparative Analysis and Functional Implications
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790, 0.97940, 0.9500, 1.0448 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 175.303, 175.303, 175.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.000 - 2.600 |
| Rwork | 0.223 |
| R-free | 0.25100 |
| Structure solution method | MAD |
| RMSD bond length | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0.36) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.062 | 0.159 |
| Number of reflections | 47553 | |
| <I/σ(I)> | 4.7 | 4.3 |
| Completeness [%] | 90.8 | 75.5 |
| Redundancy | 2.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.4 M SODIUM ACETATE 0.1 M SODIUM CACODYLATE, PH 6.5 |
