1EAO

THE RUNX1 Runt domain at 1.4A resolution: a structural switch and specifically bound chloride ions modulate DNA binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-04-15
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	91.066, 46.173, 62.904
Unit cell angles	90.00, 91.94, 90.00

Refinement procedure

Resolution	24.000 * - 1.400
R-factor	0.143
Rwork	0.141
R-free	0.17200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EBI-8233
RMSD bond length	0.015 *
RMSD bond angle	1.720 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.27)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.079	0.230
Total number of observations	224261 *
Number of reflections	50436
<I/σ(I)>	10.9	4
Completeness [%]	97.5	97 *
Redundancy	4.45	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		25 % PEG 3350, 16% GLYCEROL, 130 MM NA CACODYLATE, PH 6.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		1.6 (M)
2	1	drop		100 (mM)
3	1	drop	HEPES	20 (mM)	pH8.0
4	1	drop	protein	23 (mg/ml)
5	1	reservoir	PEG3350	25 (%(w/v))
6	1	reservoir	glycerol	16 (%(v/v))
7	1	reservoir	sodium cacodylate	130 (mM)	pH6.4