1EAG

Secreted aspartic proteinase (SAP2) from Candida albicans complexed with A70450

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	76.200, 76.200, 126.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.195 *
Rwork	0.195
R-free	0.26800
RMSD bond length	0.010
RMSD bond angle	0.035
Data reduction software	DENZO
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.050 *
Rmerge	0.072
Number of reflections	24302
Completeness [%]	98.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	20 *	Cutfield, S., (1993) J.Mol.Biol., 234, 1266. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	2.2 (M)
3	1	reservoir	sodium chloride	0.5 (M)
4	1	reservoir	Tris-HCl	20 (mM)
5	1	reservoir	sodium citrate	50 (mM)