1EAF

ATOMIC STRUCTURE OF THE CUBIC CORE OF THE PYRUVATE DEHYDROGENASE MULTIENZYME COMPLEX

Experimental procedure

Spacegroup name	F 4 3 2
Unit cell lengths	224.770, 224.770, 224.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.300 *
R-factor	0.198
Rwork	0.198
RMSD bond length	0.014
RMSD bond angle	25.500 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.094 *
Number of reflections	21825 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *		used macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	16 (%sat)