1EA9

Cyclomaltodextrinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Spacegroup name	F 2 3
Unit cell lengths	334.608, 334.608, 334.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.200
R-factor	0.214
Rwork	0.214
R-free	0.25600 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.226
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	3.200
Rmerge	0.063	0.178 *
Total number of observations	671314 *
Number of reflections	51030 *
<I/σ(I)>	12.29
Completeness [%]	90.2	85.8 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	22 *	pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH7.5
3	1	drop	beta-mercaptoethanol	1 (mM)
4	1	reservoir	PEG200	50 (%)
5	1	reservoir	HEPES	0.1 (M)	pH7.0