1EA7
Sphericase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.280, 62.380, 46.120 |
| Unit cell angles | 90.00, 114.31, 90.00 |
Refinement procedure
| Resolution | 42.300 - 0.930 |
| R-factor | 0.097 |
| R-free | 0.11640 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.261 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 0.980 |
| High resolution limit [Å] | 0.930 | 0.930 |
| Rmerge | 0.048 | 0.210 |
| Total number of observations | 934844 * | |
| Number of reflections | 130213 | |
| <I/σ(I)> | 8.1 | 1.5 |
| Completeness [%] | 97.0 | 53 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 24% PEG 6000, 5-25MM CACL2, 0.1 M HEPES, PH 7.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | reservoir | 5-25 (mM) | ||
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 7 | 1 | reservoir | PEG6000 | 24 (%) |
