1EA4

TRANSCRIPTIONAL REPRESSOR COPG/22bp dsDNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	213.400, 76.040, 50.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.950
R-factor	0.23
Rwork	0.230
R-free	0.30700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b01
RMSD bond length	0.009
RMSD bond angle	1.340
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.850
High resolution limit [Å]	2.920	2.920 *
Rmerge	0.106	0.471 *
Total number of observations	103432 *
Number of reflections	17592
<I/σ(I)>	5.9
Completeness [%]	93.6	58.6 *
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	4 *	MPD, NACL, NAACO, pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	MPD	30 (%(w/v))
3	1	reservoir		0.2 (M)
4	1	reservoir	sodium acetate	0.1 (M)