1E9S
Bacterial conjugative coupling protein TrwBdeltaN70. Unbound monoclinic form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.400, 153.400, 162.500 |
Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | TRIGONAL CRYSTAL FORM |
RMSD bond length | 0.008 |
RMSD bond angle | 1.387 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.054 |
Number of reflections | 728622 |
<I/σ(I)> | 9.9 |
Completeness [%] | 97.0 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 0.9 M POTASSIUM/SODIUM, 0.1 M HEPES PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | potassium/sodium tartrate | 0.9 (M) | |
2 | 1 | drop | HEPES | 0.1 (M) |