1E9R
Bacterial conjugative coupling protein TrwBdeltaN70. Trigonal form in complex with sulphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 151.300, 151.300, 258.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.393 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 72.600 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.057 |
Number of reflections | 1205165 |
<I/σ(I)> | 9 |
Completeness [%] | 100.0 |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 1.5 M AMMONIUM SULPHATE, 0.1 M NACL, 0.1 M HEPES PH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ammonium sulfate | 1.5 (M) | |
2 | 1 | drop | 0.1 (M) | ||
3 | 1 | drop | HEPES | 0.1 (M) |