1E9P
Crystal structure of bovine Cu, Zn SOD to 1.7 Angstrom (3 of 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.800, 51.100, 148.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.000 - 1.700 |
| Rwork | 0.185 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cbj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.065 | 0.300 |
| Total number of observations | 104800 * | |
| Number of reflections | 40372 * | |
| <I/σ(I)> | 9.8 | 2.7 |
| Completeness [%] | 91.2 | 91 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 17 (%(w/v)) | |
| 2 | 1 | reservoir | sodium chloride | 100 (mM) |
