1E9M
Ferredoxin VI from Rhodobacter Capsulatus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-15 |
| Detector | ANL-ECT |
| Wavelength(s) | 1.741,1.9815 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.340, 49.030, 54.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.270 - 2.070 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.161 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.053 * | 0.300 * |
| Number of reflections | 16751 | |
| <I/σ(I)> | 24.9 | 3.7 |
| Completeness [%] | 98.8 | 93.7 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 288 | 7 M SODIUM FORMATE, IMIDAZOLE PH 7.6, 15 DEG SEEDING IN 3.5-4.2 SODIUM FORMATE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | imidazole | 100 (mM) | |
| 3 | 1 | drop | formate | 5.4 (M) | |
| 4 | 1 | reservoir | sodium formate | 7.0 (M) | |
| 5 | 1 | reservoir | imidazole | 100 (mM) |
