1E9L
The crystal structure of novel mammalian lectin Ym1 suggests a saccharide binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.340, 60.660, 60.760 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.27200 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 27.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.039 | 0.201 * |
| Total number of observations | 46811 * | |
| Number of reflections | 12671 * | |
| <I/σ(I)> | 15.3 | 5.15 |
| Completeness [%] | 97.3 * | 95.5 * |
| Redundancy | 3.69 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6 | Change, N.C., (2001) J.Biol.Chem., 276, 17497. * |
