1E9I
Enolase from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.883, 150.006, 127.411 |
| Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
| Resolution | 27.970 - 2.480 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.970 | 2.560 |
| High resolution limit [Å] | 2.480 | 2.470 |
| Rmerge | 0.053 | 0.107 |
| Number of reflections | 63664 | 6458 * |
| <I/σ(I)> | 33 | 10 |
| Completeness [%] | 98.3 | 90 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 20 * | 20% PEG3350, 0.2M MG ACETATE, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enolase | 35 (mg/ml) | |
| 2 | 1 | drop | PEG3350 | 10 (%(w/v)) | |
| 3 | 1 | drop | magnesium acetate | 100 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 20 (%) | |
| 5 | 1 | reservoir | magnesium acetate | 200 (mM) |
