1E9I

Enolase from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-15
Detector	MAR scanner 345 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	107.883, 150.006, 127.411
Unit cell angles	90.00, 109.18, 90.00

Refinement procedure

Resolution	27.970 - 2.480
R-factor	0.229
Rwork	0.229
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ebg
RMSD bond length	0.007
RMSD bond angle	22.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.970	2.560
High resolution limit [Å]	2.480	2.470
Rmerge	0.053	0.107
Number of reflections	63664	6458 *
<I/σ(I)>	33	10
Completeness [%]	98.3	90
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	20 *	20% PEG3350, 0.2M MG ACETATE, pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enolase	35 (mg/ml)
2	1	drop	PEG3350	10 (%(w/v))
3	1	drop	magnesium acetate	100 (mM)
4	1	reservoir	PEG3350	20 (%)
5	1	reservoir	magnesium acetate	200 (mM)