1E96

Structure of the Rac/p67phox complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-04-15
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	83.220, 83.220, 138.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.400
R-factor	0.248
Rwork	0.244
R-free	0.28400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mh1 1a17
RMSD bond length	0.010
RMSD bond angle	1.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.450
High resolution limit [Å]	2.400	2.400
Rmerge	0.084	0.450
Total number of observations	123873 *
Number of reflections	20975 *
<I/σ(I)>	16.9	4.2
Completeness [%]	94.0	100
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	18 *	0.1 M CAPS PH 10.5, 0.2 M LI ACETATE, 0.8 M NAH2PO4, 1.2 M K2HPO4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop		5 (mM)
6	1	reservoir	Caps	0.1 (M)
7	1	reservoir	lithium acetate	0.2 (M)
8	1	reservoir	sodium phophate	0.8 (M)
9	1	reservoir	potassium phosphate	1.2 (M)