1E8V

Structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Spacegroup name	P 61
Unit cell lengths	137.460, 137.460, 116.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.232
Rwork	0.232
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.380
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Rmerge	0.052	0.262 *
Total number of observations	498619 *
Number of reflections	38673
Completeness [%]	94.0	98 *
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *		pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	sialic acid	10 (mM)
3	1	reservoir	PEG3350	20 (%)
4	1	reservoir	HEPES	0.1 (M)