1E8S
Alu domain of the mammalian SRP (potential Alu retroposition intermediate)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.7757,1.7753,1.033,0.9326 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 143.330, 143.330, 60.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.460 - 4.000 |
| R-factor | 0.388 |
| Rwork | 0.388 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.100 * |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 5053 | |
| <I/σ(I)> | 5.9 | 1.5 |
| Completeness [%] | 90.3 | 59.6 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 12 * | 50MM HEPES, 10MM MGCL2, 150MM NACL, 0.8 MM EU(NO3)3, 390MM (NH4)2SO4, 23% PEG400, pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SA88 RNP | 0.070 (mM) | |
| 10 | 1 | reservoir | 10 (mM) | pH7.0 | |
| 11 | 1 | reservoir | 150 (mM) | ||
| 12 | 1 | reservoir | Eu(NO3)3 | 0.80 (mM) | |
| 13 | 1 | reservoir | ammonium sulfate | 390 (mM) | |
| 14 | 1 | reservoir | PEG400 | 23 (%) | |
| 2 | 1 | drop | HEPES | 28 (mM) | pH7.0 |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 75 (mM) | ||
| 5 | 1 | drop | Eu(NO3)3 | 0.40 (mM) | |
| 6 | 1 | drop | dithiothreitol | 5 (mM) | |
| 7 | 1 | drop | ammonium sulfate | 210 (mM) | |
| 8 | 1 | drop | PEG400 | 12 (%) | |
| 9 | 1 | reservoir | HEPES | 50 (mM) |
