1E8G
STRUCTURE OF THE H61T DOUBLE MUTANT OF VANILLYL-ALCOHOL OXIDASE IN COMPLEX WITH FLUORO-CRESOL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-02-15 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 130.288, 130.288, 132.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vao |
RMSD bond length | 0.010 |
RMSD bond angle | 0.036 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.250 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.297 |
Total number of observations | 151791 * | |
Number of reflections | 57201 | |
<I/σ(I)> | 6.2 | 2.7 |
Completeness [%] | 98.9 | 99.7 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.1 * | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 4 (%(w/v)) | |
2 | 1 | reservoir | sodium acetate/HCl | 100 (mM) |