1E87
Human CD69 - trigonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.424, 48.424, 119.886 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.500 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.24400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e8i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 * | 0.106 * |
| Total number of observations | 151066 * | |
| Number of reflections | 26542 | |
| <I/σ(I)> | 33.5 | 15.6 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 * | pH 5.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 15 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | zinc sulfate | 150 (mM) | or sodium sulfate |
| 6 | 1 | reservoir | PEG6000 | 15 (%) |
