1E7N

The N-terminal domain of beta-B2-crystallin resembles the putative ancestral homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY
Synchrotron site	Photon Factory
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	110.960, 110.960, 29.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.890 - 2.350
R-factor	0.212
Rwork	0.212
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.260 *
Phasing software	CNS (0.9)
Refinement software	CNS (0.9)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.350
Rmerge	0.050 *
Number of reflections	9079
Completeness [%]	99.8
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	magnesium acetate tetrahydrate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)
4	1	reservoir	PEG8000	15 (%(w/v))