1E7D

Endonuclease VII (ENDOVII) Ffrom Phage T4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-01-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	60.900, 37.400, 74.000
Unit cell angles	90.00, 108.20, 90.00

Refinement procedure

Resolution	27.000 * - 2.800
R-factor	0.245 *
Rwork	0.245
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e7l
RMSD bond length	0.011 *
RMSD bond angle	2.300 *
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.281	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.096 *	0.283 *
Total number of observations	28458 *
Number of reflections	8966
<I/σ(I)>	7.2	3.2
Completeness [%]	99.8	99.7
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	4 *	HANGING DROP VAPOUR DIFFUSION DROP SIZE: 1 + 1 UL PROTEIN SOLUTION: 16 MG/ML ENDOVII, 175 MM NACL, 20 MM MGCL2, 2 MM ZNCL2, 10 MM 2-MERCAPTO-ETHANOL, 10 % GLYCEROL, 10 MM MOPS PH6.5. WELL: 16-18% PEG 5000 MME, 200 MM CACL2, 20 MM AMMONIUM SULPHATE, 10 MM 2-MERCAPTO-ETHANOL, 100 MM TRIS PH 8.2, C.A. 1 MM SODIUM AZIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	reservoir	PEG2000 MME	18 (%(w/v))
3	1	reservoir	Tris	100 (mM)
4	1	reservoir		200 (mM)
5	1	reservoir	ammonium sulfate	5 (mM)
6	1	reservoir		5 (mM)
7	1	reservoir	beta-mercaptoethanol	10 (mM)