1E78

Crystal structure of human serum albumin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	298
Detector technology	CCD
Collection date	1999-10-15
Detector	ADSC
Spacegroup name	P 1
Unit cell lengths	54.840, 55.620, 120.270
Unit cell angles	81.22, 91.08, 64.28

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.247
Rwork	0.247
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ao6
RMSD bond length	0.006
RMSD bond angle	22.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.300	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.045	0.251
Number of reflections	37956
<I/σ(I)>	4	1.3
Completeness [%]	97.5	97.3
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7	4 *	pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	28-30 (%(w/v))
2	1	reservoir	potassium phosphate	50 (mM)