1E73
2-F-glucosylated MYROSINASE FROM SINAPIS ALBA with bound L-ascorbate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-10-15 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 135.300, 137.200, 80.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
R-factor | 0.134 * |
Rwork | 0.134 |
R-free | 0.16700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e4m |
RMSD bond length | 0.021 |
RMSD bond angle | 0.036 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | X-PLOR |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.700 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.063 | 0.352 |
Number of reflections | 98776 | |
<I/σ(I)> | 8 | 2.1 |
Completeness [%] | 82.7 | 71.4 |
Redundancy | 4.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | Burmeister, W.P., (1997) Structure (London), 5, 663. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | myrosinase | 12 (mg/ml) | |
2 | 1 | drop | HEPES | 30 (mM) | |
3 | 1 | drop | 0.05 (%) | ||
4 | 1 | reservoir | ammonium sulfate | 66 (%sat) | |
5 | 1 | reservoir | Tris-HCl | 100 (mM) |