1E6U

GDP 4-keto-6-deoxy-D-mannose epimerase reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	102.800, 102.800, 74.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.450
Rwork	0.135
R-free	0.16700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bws
RMSD bond length	0.015
RMSD bond angle	0.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.057
Number of reflections	80994
<I/σ(I)>	14.8	5.5
Completeness [%]	99.1	99
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	21 *	1.5 M LITHIUM SULPHATE, PH 6.5 0.1M TRIS BUFFER 21C

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	11-20 (mg/ml)
2	1	2	lithium sulfate	1.5 (M)
3	1	2	MES	0.1 (M)	or Tris