1E6K
Two-component signal transduction system D12A mutant of CheY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.100, 54.300, 60.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.557 |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.000 | 2.000 * |
| Rmerge | 0.064 * | |
| Total number of observations | 29441 * | |
| Number of reflections | 7957 * | |
| Completeness [%] | 92.3 | 85.3 * |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.6 (mg/ml) | |
| 2 | 1 | drop | magnesium acetate | 0.1 (M) | |
| 3 | 1 | drop | PEG8000 | 10 (%) | |
| 4 | 1 | drop | cacodylate | 50 (mM) | |
| 5 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 6 | 1 | reservoir | PEG8000 | 20 (%) | |
| 7 | 1 | reservoir | cacodylate | 100 (mM) |
