1E6H
A-SPECTRIN SH3 DOMAIN A11V, M25I, V44I, V58L MUTANTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE M18X |
| Temperature [K] | 100 |
| Collection date | 2000-04-15 |
| Detector | SMALL MARRESEARCH IMAGING PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.040, 43.590, 53.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.010 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.788 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.050 |
| Rmerge | 0.044 | 0.079 |
| Number of reflections | 3830 | |
| Completeness [%] | 87.4 | 78 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 20 * | PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH=6.0, 90 MM BIS-TRIS PROPANE, 0.9 MM EDTA, 0.9 MM DTT, 0.9 MM SODIUM AZIDE, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG4000 | ||
| 2 | 1 | 1 | ammonium sulfate |
