1E6F

Human MIR-receptor, repeat 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	48.500, 49.000, 120.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.750
R-factor	0.2209
R-free	0.27270
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.021
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	1.850
High resolution limit [Å]	1.753	1.750
Rmerge	0.025	0.089 *
Number of reflections	29383
<I/σ(I)>	40.8	9.9
Completeness [%]	99.1	97.4 *
Redundancy	8.3 *	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	288 *	PRECIPITANT: 0.2 M AMMONIUM ACETATE, 0.1 M CACODYLATE PH 5 28% PEG 4000. PROTEIN SOLUTION: 8 MG/ML IN 10 MM TRIS-HCL PH7.5, 150 MM N VAPOUR DIFFUSION, HANGING DROPS,1:1 RATIO.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		150 (mM)
4	1	drop	PEG4000	28 (%)
5	1	drop	ammonium acetate	0.2 (M)
6	1	reservoir	cacodylate	100 (mM)