1E6D

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH TRP M115 REPLACED WITH PHE (CHAIN M, WM115F) PHE M197 REPLACED WITH ARG (CHAIN M, FM197R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-10-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	141.200, 141.200, 187.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.400 - 2.300
R-factor	0.174 *
Rwork	0.174
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES (UNPUBLISHED DATA)
RMSD bond length	0.014
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.400	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.110	0.530
Number of reflections	90885
Completeness [%]	94.5	71.2
Redundancy	3.8	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	lauryldimethylamine oxide	0.09 (%(v/v))
3	1	drop	1.2.3-heptanetriol	3.5 (%(w/v))
4	1	drop	potassium phosphate	0.75 (M)
5	1	reservoir	potassium phosphate	1.5 (M)