1E69
SMC head domain from Thermotoga maritima
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-15 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93, 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.000, 49.190, 233.880 |
Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
Resolution | 100.000 - 3.100 |
R-factor | 0.25 |
Rwork | 0.250 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.395 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.071 * | 0.306 |
Number of reflections | 45940 | |
<I/σ(I)> | 11.4 | 3 |
Completeness [%] | 97.3 | 97.3 |
Redundancy | 2.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 5.6 | pH 5.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | reservoir solution | ||
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
5 | 1 | reservoir | PEG6000 | 22 (%) | |
6 | 1 | reservoir | urea | 0.013 (mM) |