1E67
Zn-Azurin from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1991-06-10 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.490, 80.650, 110.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.140 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| Structure solution method | OTHER |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.900 |
| Data reduction software | MADNESS |
| Data scaling software | ABSCOR |
| Phasing software | X-PLOR (2.1) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.109 | |
| Number of reflections | 26009 | |
| Completeness [%] | 79.1 | 44.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9 * | Nar, H., (1991) J.Mol.Biol., 221, 765. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 3 | 1 | drop | lithium nitrate | 0.1 (M) | |
| 4 | 1 | drop | acetate | 0.1 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 4.0 (M) | |
| 6 | 1 | reservoir | lithium nitrate | 0.7 (M) | |
| 7 | 1 | reservoir | acetate | 0.1 (M) |
