1E64
FERREDOXIN:NADP+ REDUCTASE MUTANT WITH LYS 75 REPLACED BY GLN (K75Q)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-15 |
Detector | MARRESEARCH |
Spacegroup name | P 65 |
Unit cell lengths | 88.130, 88.130, 97.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.300 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1que |
RMSD bond length | 0.008 |
RMSD bond angle | 26.753 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.500 | 2.440 |
High resolution limit [Å] | 2.300 | 2.310 |
Rmerge | 0.080 | 0.207 |
Number of reflections | 18682 | |
<I/σ(I)> | 7.6 | 3.6 |
Completeness [%] | 99.2 | 95.1 |
Redundancy | 6.9 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 5.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.75 (mM) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | beta-octylglucoside | 5 (%(w/v)) | |
4 | 1 | reservoir | PEG6000 | 18-20 (%(w/v)) | |
5 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
6 | 1 | reservoir | MES- NaOH | 0.1 (M) |