1E5J
ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN THE TETRAGONAL CRYSTAL FORM IN COMPLEX WITH METHYL-4II-S-ALPHA-CELLOBIOSYL-4II-THIO-BETA-CELLOBIOSIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-01-13 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.520, 74.520, 136.193 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.850 |
R-factor | 0.177 * |
Rwork | 0.177 |
R-free | 0.22100 * |
Structure solution method | OTHER |
RMSD bond length | 0.013 |
RMSD bond angle | 0.023 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.048 | 0.265 |
Number of reflections | 32666 | |
<I/σ(I)> | 19.8 | 3.7 |
Completeness [%] | 98.7 | 96.5 |
Redundancy | 3.0 * | 3.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 100 * | Varrot, A., (2000) J.Mol.Biol., 279, 819. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG400 | 28 (%) | |
3 | 1 | reservoir | triethanolamine | 100 (mM) | |
4 | 1 | reservoir | 200 (mM) |