1E5D
RUBREDOXIN OXYGEN:OXIDOREDUCTASE (ROO) FROM ANAEROBE DESULFOVIBRIO GIGAS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.240, 101.250, 90.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
R-factor | 0.1618 * |
R-free | 0.24770 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.019 |
Data reduction software | HKL |
Data scaling software | HKL |
Phasing software | SHELXL-97 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.066 | 0.265 |
Total number of observations | 123925 * | |
Number of reflections | 31676 | |
<I/σ(I)> | 17.5 | 4.7 |
Completeness [%] | 99.6 | 96.9 * |
Redundancy | 3.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 4-10 * | VAPOUR DIFFUSION IN SITTING DROP. DROPS OF PROTEIN SOLUTION 10MG/ML AND ALIQUOTS OF PRECIPITANT SOLUTION COMPOSED OF PEG 6 K 10%, TRIS-MALEIC BUFFER 0.1 M PH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG6000 | 10 (%(v/v)) | |
3 | 1 | reservoir | Tris-maleate | 100 (mM) | pH6.0 |