1E57
PHYSALIS MOTTLE VIRUS: EMPTY CAPSID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 289.640, 287.722, 295.226 |
| Unit cell angles | 63.59, 61.39, 62.94 |
Refinement procedure
| Resolution | 10.000 - 3.200 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 27.000 * |
| Data reduction software | XDS |
| Data scaling software | MARSCALE |
| Phasing software | GLRF |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.149 |
| Number of reflections | 605968 |
| Completeness [%] | 36.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | PH 5.6 SODIUM ACETATE; WITH 20% PEG AS PRECIPITANT. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 80-100 (mg/ml) | |
| 2 | 1 | 1 | sodium acetate | 0.5 (M) | |
| 3 | 1 | 1 | dithiothreitol | 10 (mM) | |
| 4 | 1 | 1 | 2 (mM) | ||
| 5 | 1 | 1 | PEG8000 | 2-4 (%(w/v)) |
