1E51
Crystal structure of native human erythrocyte 5-aminolaevulinic acid dehydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 125.530, 125.530, 200.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.380 - 2.830 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aw5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.233 | 2.980 |
| High resolution limit [Å] | 2.832 | 2.830 |
| Rmerge | 0.071 | 0.257 |
| Number of reflections | 18652 | |
| <I/σ(I)> | 6 | 2.9 |
| Completeness [%] | 96.0 | 76.7 |
| Redundancy | 5.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | MES, AMMONIUM SULPHATE, DIOXANE, pH 6.20 |
