1E4V

Mutant G10V of adenylate kinase from E. coli, modified in the Gly-loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	279
Spacegroup name	P 21 2 21
Unit cell lengths	73.200, 79.800, 85.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.850
R-factor	0.196
Rwork	0.196
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	3.400
Phasing software	X-PLOR
Refinement software	X-PLOR (1.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.400
Rmerge	0.100
Number of reflections	20297
Completeness [%]	90.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7	20 *	pH 6.70

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-25 (mg/ml)
2	1	drop	Ap5A	2 (mM)
3	1	drop	MES	50 (mM)
4	1	drop	PEG1500	1.5 (%(w/v))
5	1	drop	ammonium sulfate	1.5 (M)
6	1	reservoir	MES	50 (mM)
7	1	reservoir	ammonium sulfate	2.0-2.2 (M)