1E4L

Crystal structure of the inactive mutant Monocot (Maize ZMGlu1) beta-glucosidase ZM Glu191Asp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Collection date	2000-03-15
Spacegroup name	P 21 21 21
Unit cell lengths	92.070, 94.920, 117.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 2.200
R-factor	0.207
Rwork	0.207
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e1e
RMSD bond length	0.007
RMSD bond angle	22.500 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.043	0.141
Number of reflections	549492
<I/σ(I)>	14.2	2.4
Completeness [%]	98.3	96.4
Redundancy	3.1	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		22 % PEG 4000, 5 % ISOPROPANOL, 0.1 M HEPES PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)
2	1	reservoir	PEG4000	22 (%)
3	1	reservoir	isopropanol	5 (%)