1E4K
CRYSTAL STRUCTURE OF SOLUBLE HUMAN IGG1 FC FRAGMENT-FC-GAMMA RECEPTOR III COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 115.320, 115.320, 299.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 3.200 |
| R-factor | 0.2708 |
| Rwork | 0.271 |
| R-free | 0.35690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2FCB 1fc1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.170 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.133 | 0.607 * |
| Number of reflections | 18596 | |
| Completeness [%] | 94.9 | 66.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.1 * | 18 * | 0.1M MES/TRIS PH 5.6, 0.2M K/NA TARTRATE, 6.0% PEG8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Mops | 2 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | sodium azide | 0.02 (%) | |
| 5 | 1 | reservoir | MES-Tris | 100 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 6 (%(w/v)) | |
| 7 | 1 | reservoir | K-Na tartrate | 200 (mM) | |
| 8 | 1 | reservoir | sodium azide | 0.02 (%) |
