1E4D

Structure of OXA10 beta-lactamase at pH 8.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	67.279, 82.436, 101.165
Unit cell angles	90.00, 95.89, 90.00

Refinement procedure

Resolution	29.540 - 1.800
R-factor	0.1983 *
Rwork	0.198
R-free	0.24271
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e3u
RMSD bond length	0.005
RMSD bond angle	0.018
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.540	1.890
High resolution limit [Å]	1.800	1.800
Rmerge	0.086 *	0.331 *
Total number of observations	258171 *
Number of reflections	86153
<I/σ(I)>	12.3	2.7
Completeness [%]	84.4	91.1 *
Redundancy	3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	4 *	AMMONIUMS SULFATE 2.0 M, TRIS HCL 100 MM, PH 8.2-8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium potassium phosphate	20 (mM)
3	1	reservoir	ammonium sulfate	2.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)