1E4D
Structure of OXA10 beta-lactamase at pH 8.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.279, 82.436, 101.165 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 29.540 - 1.800 |
| R-factor | 0.1983 * |
| Rwork | 0.198 |
| R-free | 0.24271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3u |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.018 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 * | 0.331 * |
| Total number of observations | 258171 * | |
| Number of reflections | 86153 | |
| <I/σ(I)> | 12.3 | 2.7 |
| Completeness [%] | 84.4 | 91.1 * |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 * | 4 * | AMMONIUMS SULFATE 2.0 M, TRIS HCL 100 MM, PH 8.2-8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium potassium phosphate | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) |
